PrimePCR™ Probe Assay: Faf1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0045278
Assay Design:   Intron-spanning
Chromosome Location:   5:133436138-133453538question
Amplicon Length:   90
Splice Variants Targeted:   ENSRNOT00000011765

Gene Information

human homolog is a member of a protein complex involved in Fas-mediated apoptosis; plays a role in negative regulation of NF-kappaB activation [RGD, Feb 2006]

Gene Symbol:   Faf1
Gene Name:   FAS-associated factor 1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000008523
Entrez:   140657
UniGene:   Rn.198288
Chromosome Mapping:   5q35

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 37.200000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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