PrimePCR™ Probe Assay: No symbol available for ENSRNOG00000047540, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: No symbol available for ENSRNOG00000047540, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: No symbol available for ENSRNOG00000047540, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0042485
Assay Design:   intron_spanning
Chromosome Location:   1:70648683-70650211question
Amplicon Length:   77
Splice Variants Targeted:   ENSRNOT00000021263 ENSRNOT00000066583 ENSRNOT00000024704 ENSRNOT00000064347 ENSRNOT00000028786 ENSRNOT00000058616 ENSRNOT00000007110 ENSRNOT00000072183 ENSRNOT00000001750 ENSRNOT00000016415 ENSRNOT00000001081 ENSRNOT00000007940 ENSRNOT00000016423 ENSRNOT00000033222 ENSRNOT00000058419 ENSRNOT00000016479 ENSRNOT00000018185 ENSRNOT00000016628 ENSRNOT00000019096 ENSRNOT00000037177 ENSRNOT00000005942 ENSRNOT00000073066 ENSRNOT00000056014

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000047540
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000047540
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.940000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download