PrimePCR™ Probe Assay: Wdr64, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    RMB0.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0038200
Assay Design:   intron_spanning
Chromosome Location:   13:98292213-98294744question
Amplicon Length:   158
Splice Variants Targeted:   ENSRNOT00000073225 ENSRNOT00000065706 ENSRNOT00000038306 ENSRNOT00000038285 ENSRNOT00000007461

Gene Information

Description Not Available

Gene Symbol:   Wdr64
Gene Name:   Protein Wdr64
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000038083
Entrez:   689524
UniGene:   Not Available
Chromosome Mapping:   13q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 37.280000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download