PrimePCR™ Probe Assay: Adam22, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Adam22, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Adam22, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0032623
Assay Design:   intron_spanning
Chromosome Location:   4:22822881-22825201question
Amplicon Length:   181
Splice Variants Targeted:   ENSRNOT00000073595 ENSRNOT00000070872 ENSRNOT00000071921 ENSRNOT00000072068 ENSRNOT00000073723 ENSRNOT00000073501 ENSRNOT00000074918 ENSRNOT00000073044 ENSRNOT00000074832 ENSRNOT00000068410 ENSRNOT00000071635

Gene Information

metalloprotease-disintegrin containing a catalytic zinc ion with moderate sequence similarity to human metalloproteinase-disintegrin domain 22 (MDC2 delta), isoform 1 preproprotein [RGD, Feb 2006]

Gene Symbol:   Adam22
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000042478
Entrez:   57033
Chromosome Mapping:   4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 36.190000
Efficiency 106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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