PrimePCR™ Probe Assay: Vhl, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0028807
Assay Design:   intron_spanning
Chromosome Location:   4:208880823-208881955question
Amplicon Length:   84
Splice Variants Targeted:   ENSRNOT00000013727

Gene Information

may act as a tumor suppressor [RGD, Feb 2006]

Gene Symbol:   Vhl
Gene Name:   Von Hippel-Lindau disease tumor suppressor
Aliases:   Not Available
RefSeq:   NM_052801
Ensembl:   ENSRNOG00000010258
Entrez:   24874
UniGene:   Rn.11059
Chromosome Mapping:   4q41.3-q42.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 37.810000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download