PrimePCR™ Probe Assay: Galnt10, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0028569
Assay Design:   intron_spanning
Chromosome Location:   10:42959185-42988810question
Amplicon Length:   63
Splice Variants Targeted:   ENSRNOT00000003447

Gene Information

UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase; catalyzes the transfer of GalNAc from the nucleotide sugar UDP-GalNAc to the hydroxyl group of either serine or threonine [RGD, Feb 2006]

Gene Symbol:   Galnt10
Gene Name:   Polypeptide N-acetylgalactosaminyltransferase 10
Aliases:   Not Available
RefSeq:   NM_130742
Ensembl:   ENSRNOG00000002488
Entrez:   170501
UniGene:   Rn.60224
Chromosome Mapping:   10q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.090000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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