PrimePCR™ Probe Assay: Pparg, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0027867
Assay Design:   intron_spanning
Chromosome Location:   4:210671704-210688184question
Amplicon Length:   133
Splice Variants Targeted:   ENSRNOT00000001298 ENSRNOT00000072952 ENSRNOT00000051858 ENSRNOT00000012137

Gene Information

ligand-activated transcription factor; mediates expression of genes involved in lipid metabolism [RGD, Feb 2006]

Gene Symbol:   Pparg
Gene Name:   Peroxisome proliferator-activated receptor gamma
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000008839
Entrez:   25664
UniGene:   Rn.23443
Chromosome Mapping:   4q42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.650000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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