PrimePCR™ Probe Assay: Pdcd4, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0023905
Assay Design:   intron_spanning
Chromosome Location:   1:282053719-282055165question
Amplicon Length:   101
Splice Variants Targeted:   ENSRNOT00000022861 ENSRNOT00000020155 ENSRNOT00000059153

Gene Information

acts as an inhibitor of apoptosis; has similarity to eukaryotic translation initiation factor (eIF)4G [RGD, Feb 2006]

Gene Symbol:   Pdcd4
Gene Name:   Programmed cell death protein 4
Aliases:   Not Available
RefSeq:   NM_022265
Ensembl:   ENSRNOG00000014779
Entrez:   64031
UniGene:   Rn.122017
Chromosome Mapping:   1q55

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.420000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download