PrimePCR™ Probe Assay: No symbol available for ENSRNOG00000050724, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0049020
Assay Design:   Exonic
Chromosome Location:   2:211862438-211862614question
Amplicon Length:   147
Splice Variants Targeted:   ENSRNOT00000001642 ENSRNOT00000018932 ENSRNOT00000012691 ENSRNOT00000065884 ENSRNOT00000075788 ENSRNOT00000072995 ENSRNOT00000065242 ENSRNOT00000056485 ENSRNOT00000045938 ENSRNOT00000012420 ENSRNOT00000041480 ENSRNOT00000012464 ENSRNOT00000056486

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000050724
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000050724
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 37.500000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download