PrimePCR™ Probe Assay: No symbol available for ENSRNOG00000031960, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0048212
Assay Design:   Exonic
Chromosome Location:   15:103760398-103760501question
Amplicon Length:   74
Splice Variants Targeted:   ENSRNOT00000061270 ENSRNOT00000066582 ENSRNOT00000016911 ENSRNOT00000004965 ENSRNOT00000007843 ENSRNOT00000066669 ENSRNOT00000036054 ENSRNOT00000006501 ENSRNOT00000007710 ENSRNOT00000074605 ENSRNOT00000016784 ENSRNOT00000012136 ENSRNOT00000021790 ENSRNOT00000042321 ENSRNOT00000030485 ENSRNOT00000005516 ENSRNOT00000026576 ENSRNOT00000043621 ENSRNOT00000041344 ENSRNOT00000005986

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000031960
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000031960
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.995600
y-intercept 38.370000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download