PrimePCR™ Probe Assay: No symbol available for ENSRNOG00000033635, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0044285
Assay Design:   exonic
Chromosome Location:   3:49856163-49856262question
Amplicon Length:   70
Splice Variants Targeted:   ENSRNOT00000032824 ENSRNOT00000043945 ENSRNOT00000000455 ENSRNOT00000039910 ENSRNOT00000036106 ENSRNOT00000041811 ENSRNOT00000013168 ENSRNOT00000049603 ENSRNOT00000037885 ENSRNOT00000008851 ENSRNOT00000043243 ENSRNOT00000047948 ENSRNOT00000050443 ENSRNOT00000002985 ENSRNOT00000044998 ENSRNOT00000047750 ENSRNOT00000058917 ENSRNOT00000041328 ENSRNOT00000043709 ENSRNOT00000047820 ENSRNOT00000034990

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000033635
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000033635
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.280000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download