PrimePCR™ Probe Assay: No symbol available for ENSRNOG00000050536, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: No symbol available for ENSRNOG00000050536, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: No symbol available for ENSRNOG00000050536, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0043098
Assay Design:   exonic
Chromosome Location:   1:150633497-150633637question
Amplicon Length:   111
Splice Variants Targeted:   ENSRNOT00000002851 ENSRNOT00000015272 ENSRNOT00000042938 ENSRNOT00000075610 ENSRNOT00000005623 ENSRNOT00000018550 ENSRNOT00000000041 ENSRNOT00000044896 ENSRNOT00000000313 ENSRNOT00000072139 ENSRNOT00000044477 ENSRNOT00000035438 ENSRNOT00000020291 ENSRNOT00000048506 ENSRNOT00000013608 ENSRNOT00000067118 ENSRNOT00000016947 ENSRNOT00000027187 ENSRNOT00000073123 ENSRNOT00000073674

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000050536
Gene Name:   Elongation factor 1-alpha
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000050536
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.120000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download