PrimePCR™ Probe Assay: Svs3b, Rat

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0038994
Assay Design:   exonic
Chromosome Location:   3:165290077-165290284question
Amplicon Length:   178
Splice Variants Targeted:   ENSRNOT00000022779 ENSRNOT00000039316 ENSRNOT00000073209 ENSRNOT00000010957 ENSRNOT00000042142 ENSRNOT00000066035 ENSRNOT00000028440 ENSRNOT00000055166 ENSRNOT00000045677 ENSRNOT00000063906

Gene Information

Description Not Available

Gene Symbol:   Svs3b
Gene Name:   Seminal vesicle secretory protein 3B precursor
Aliases:   Not Available
RefSeq:   NM_001102417
Ensembl:   ENSRNOG00000036782
Entrez:   296354
UniGene:   Rn.100666
Chromosome Mapping:   3q42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.810000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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