PrimePCR™ Probe Assay: RT1-M6-1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0036256
Assay Design:   exonic
Chromosome Location:   20:4099148-4099296question
Amplicon Length:   119
Splice Variants Targeted:   ENSRNOT00000040634 ENSRNOT00000051480 ENSRNOT00000041500 ENSRNOT00000037854 ENSRNOT00000016449 ENSRNOT00000060727 ENSRNOT00000037846 ENSRNOT00000060726 ENSRNOT00000010283 ENSRNOT00000008964

Gene Information

Description Not Available

Gene Symbol:   RT1-M6-1
Gene Name:   RT1 class I, locus M6, gene 1 precursor
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000022107
Entrez:   414785
UniGene:   Rn.142703
Chromosome Mapping:   20p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.190000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download