PrimePCR™ Probe Assay: Commd5, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0033504
Assay Design:   exonic
Chromosome Location:   7:117948926-117949075question
Amplicon Length:   120
Splice Variants Targeted:   ENSRNOT00000071130 ENSRNOT00000013038 ENSRNOT00000051169 ENSRNOT00000005925 ENSRNOT00000043345 ENSRNOT00000028496 ENSRNOT00000075405

Gene Information

plays a role as a regulator of renal epithelial cell growth and differentiation causing G(2)M cell cycle arrest [RGD, Feb 2006]

Gene Symbol:   Commd5
Gene Name:   COMM domain containing 5, isoform CRA_a; COMM domain-containing protein 5
Aliases:   Not Available
RefSeq:   NM_139108
Ensembl:   ENSRNOG00000050582
Entrez:   245974
UniGene:   Rn.24747
Chromosome Mapping:   7q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.590000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download