PrimePCR™ Probe Assay: Nelf, Rat

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0031801
Assay Design:   exonic
Chromosome Location:   3:2250202-2250433question
Amplicon Length:   60
Splice Variants Targeted:   ENSRNOT00000070794 ENSRNOT00000045515 ENSRNOT00000067442 ENSRNOT00000009008 ENSRNOT00000016281 ENSRNOT00000015181 ENSRNOT00000061303 ENSRNOT00000048353 ENSRNOT00000042100 ENSRNOT00000012129

Gene Information

mouse homolog acts as a guidance molecule for olfactory axon projections and is involved in the neurophilic migration of LHRH cells [RGD, Feb 2006]

Gene Symbol:   Nelf
Gene Name:   Nasal embryonic luteinizing hormone-releasing hormone factor isoform 1
Aliases:   Not Available
RefSeq:   NM_057190
Ensembl:   ENSRNOG00000008810
Entrez:   117536
UniGene:   Rn.7504
Chromosome Mapping:   3p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.994900
y-intercept 36.110000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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