PrimePCR™ Probe Assay: Mrgprb5, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0031261
Assay Design:   exonic
Chromosome Location:   1:104605557-104605684question
Amplicon Length:   98
Splice Variants Targeted:   ENSRNOT00000029958

Gene Information

seven transmembrane domain G-protein coupled receptor; most often found in sensory neurons [RGD, Feb 2006]

Gene Symbol:   Mrgprb5
Gene Name:   Mas-related G-protein coupled receptor member B5
Aliases:   Not Available
RefSeq:   NM_001002284
Ensembl:   ENSRNOG00000022703
Entrez:   404644
UniGene:   Rn.141968
Chromosome Mapping:   1q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 37.090000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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