PrimePCR™ Probe Assay: Nos1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0031132
Assay Design:   exonic
Chromosome Location:   12:46209420-46209569question
Amplicon Length:   120
Splice Variants Targeted:   ENSRNOT00000060696 ENSRNOT00000066810 ENSRNOT00000001493 ENSRNOT00000046205

Gene Information

enzyme that catalyzes the production of nitric oxide [RGD, Feb 2006]

Gene Symbol:   Nos1
Gene Name:   Nitric oxide synthase, brain
Aliases:   Not Available
RefSeq:   NM_052799
Ensembl:   ENSRNOG00000001130
Entrez:   24598
Chromosome Mapping:   12q16

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.460000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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