PrimePCR™ Probe Assay: Kcnj9, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0028467
Assay Design:   exonic
Chromosome Location:   13:95225316-95225430question
Amplicon Length:   85
Splice Variants Targeted:   ENSRNOT00000010113

Gene Information

G-protein activated potassium channel [RGD, Feb 2006]

Gene Symbol:   Kcnj9
Gene Name:   G protein-activated inward rectifier potassium channel 3
Aliases:   Not Available
RefSeq:   NM_053834
Ensembl:   ENSRNOG00000007645
Entrez:   116560
UniGene:   Not Available
Chromosome Mapping:   13q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.140000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download