PrimePCR™ Probe Assay: Cdkn1c, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Cdkn1c, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Cdkn1c, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0026265
Assay Design:   exonic
Chromosome Location:   1:223521224-223521406question
Amplicon Length:   153
Splice Variants Targeted:   ENSRNOT00000027831 ENSRNOT00000027885 ENSRNOT00000073862

Gene Information

cyclin-dependent protein kinase inhibitor; may be involved in regulating cell proliferation during embryogenesis [RGD, Feb 2006]

Gene Symbol:   Cdkn1c
Gene Name:   Cyclin-dependent kinase inhibitor 1C, p57 isoform 1
Aliases:   Not Available
RefSeq:   NM_182735 NM_001033758 NM_001033757
Ensembl:   ENSRNOG00000020559
Entrez:   246060
Chromosome Mapping:   1q42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.630000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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