PrimePCR™ Probe Assay: Rnase17, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Rnase17, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Rnase17, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0024930
Assay Design:   exonic
Chromosome Location:   15:32034434-32034609question
Amplicon Length:   146
Splice Variants Targeted:   ENSRNOT00000022637 ENSRNOT00000045533 ENSRNOT00000040857 ENSRNOT00000041413 ENSRNOT00000025273 ENSRNOT00000006162 ENSRNOT00000025978 ENSRNOT00000037523 ENSRNOT00000009872 ENSRNOT00000064211 ENSRNOT00000003006 ENSRNOT00000060465 ENSRNOT00000018695 ENSRNOT00000028331 ENSRNOT00000023137 ENSRNOT00000060987 ENSRNOT00000071158 ENSRNOT00000048941 ENSRNOT00000071093

Gene Information

Description Not Available

Gene Symbol:   Rnase17
Gene Name:   Ribonuclease 17 precursor
Aliases:   Not Available
RefSeq:   NM_001012232
Ensembl:   ENSRNOG00000050295
Entrez:   497195
Chromosome Mapping:   15p14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 36.270000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download