PrimePCR™ Probe Assay: Vmn1r34, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Vmn1r34, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Vmn1r34, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0060679
Assay Design:   exonic
Chromosome Location:   6:66637463-66637598question
Amplicon Length:   106
Splice Variants Targeted:   ENSMUST00000176838 ENSMUST00000096612 ENSMUST00000164307 ENSMUST00000079669 ENSMUST00000171186 ENSMUST00000176073 ENSMUST00000176376 ENSMUST00000177267 ENSMUST00000049694 ENSMUST00000168416 ENSMUST00000079584 ENSMUST00000074381 ENSMUST00000071414 ENSMUST00000177072 ENSMUST00000077482

Gene Information

Description Not Available

Gene Symbol:   Vmn1r34
Gene Name:   Vomeronasal 1 receptor 34
Aliases:   EG546901, Gm5991
RefSeq:   NC_000072.6 NT_039353.8
Ensembl:   ENSMUSG00000091012
Entrez:   546901
Chromosome Mapping:   6 C1|6

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.980000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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