PrimePCR™ Probe Assay: Eef1d, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0059343
Assay Design:   exonic
Chromosome Location:   15:75896460-75896725question
Amplicon Length:   61
Splice Variants Targeted:   ENSMUST00000099502 ENSMUST00000025520 ENSMUST00000039928 ENSMUST00000001202 ENSMUST00000115013 ENSMUST00000154584 ENSMUST00000109972 ENSMUST00000116440 ENSMUST00000137426 ENSMUST00000089681 ENSMUST00000089680 ENSMUST00000023235 ENSMUST00000144614 ENSMUST00000141268 ENSMUST00000123712 ENSMUST00000109975 ENSMUST00000028279

Gene Information

Description Not Available

Gene Symbol:   Eef1d
Gene Name:   Eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
Aliases:   1700026P12Rik, 5730529A16Rik, AL023999
RefSeq:   NC_000081.6 NT_039621.8
Ensembl:   ENSMUSG00000055762
Entrez:   66656
UniGene:   Mm.258927
Chromosome Mapping:   15|15 E1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.060000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download