PrimePCR™ Probe Assay: Syngr2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0059310
Assay Design:   exonic
Chromosome Location:   11:117813570-117813680question
Amplicon Length:   81
Splice Variants Targeted:   ENSMUST00000099775 ENSMUST00000171436 ENSMUST00000165095 ENSMUST00000056177 ENSMUST00000169350 ENSMUST00000043676 ENSMUST00000026649 ENSMUST00000177131 ENSMUST00000120928 ENSMUST00000175737

Gene Information

Description Not Available

Gene Symbol:   Syngr2
Gene Name:   Synaptogyrin 2
Aliases:   Clast2, cellugyrin
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000048277
Entrez:   20973
UniGene:   Mm.468228
Chromosome Mapping:   11 E2|11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.370000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download