PrimePCR™ Probe Assay: Sh3bgr, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0057707
Assay Design:   exonic
Chromosome Location:   16:96206514-96206610question
Amplicon Length:   67
Splice Variants Targeted:   ENSMUST00000048770 ENSMUST00000166952 ENSMUST00000132424 ENSMUST00000129904 ENSMUST00000123728 ENSMUST00000171181

Gene Information

Description Not Available

Gene Symbol:   Sh3bgr
Gene Name:   SH3-binding domain glutamic acid-rich protein
Aliases:   5430437A18Rik
RefSeq:   NC_000082.6 NT_039625.8 NT_187015.1
Ensembl:   ENSMUSG00000040666
Entrez:   50795
UniGene:   Mm.28638
Chromosome Mapping:   16 C4|16

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.730000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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