PrimePCR™ Probe Assay: St7, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0055839
Assay Design:   exonic
Chromosome Location:   6:17850424-17852387question
Amplicon Length:   102
Splice Variants Targeted:   ENSMUST00000115420 ENSMUST00000115419 ENSMUST00000052113 ENSMUST00000081635 ENSMUST00000115418 ENSMUST00000077080 ENSMUST00000000674 ENSMUST00000053148 ENSMUST00000115417

Gene Information

Description Not Available

Gene Symbol:   St7
Gene Name:   Suppression of tumorigenicity 7
Aliases:   9430001H04Rik, Fam4a2, HELG, RAY1, SEN4, TSG7
RefSeq:   NC_000072.6 NT_039340.7
Ensembl:   ENSMUSG00000029534
Entrez:   64213
UniGene:   Mm.12051
Chromosome Mapping:   6 A2|6

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.750000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download