PrimePCR™ Probe Assay: Hnf4a, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0053865
Assay Design:   exonic
Chromosome Location:   2:163558214-163558977question
Amplicon Length:   71
Splice Variants Targeted:   ENSMUST00000042666 ENSMUST00000167859 ENSMUST00000056085 ENSMUST00000006742 ENSMUST00000003433 ENSMUST00000036041 ENSMUST00000170285 ENSMUST00000032462 ENSMUST00000109411 ENSMUST00000137449 ENSMUST00000018094 ENSMUST00000169597 ENSMUST00000165978 ENSMUST00000029562

Gene Information

Description Not Available

Gene Symbol:   Hnf4a
Gene Name:   Hepatic nuclear factor 4, alpha
Aliases:   HNF-4, Hnf4, Hnf4alpha, MODY1, Nr2a1, Tcf14
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000017950
Entrez:   15378
UniGene:   Mm.202383
Chromosome Mapping:   2 H3|2 94.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.760000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download