PrimePCR™ Probe Assay: Stk10, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0053127
Assay Design:   exonic
Chromosome Location:   11:32610721-32612800question
Amplicon Length:   105
Splice Variants Targeted:   ENSMUST00000102821

Gene Information

This gene encodes a member of the Ste20 family of serine/threonine protein kinases and is similar to several known polo-like kinase kinases. Mice deficient for this gene product are viable but exhibit altered integrin-mediated lymphocyte adhesion characteristics. The orthologous gene product in humans can associate with and phosphorylate polo-like kinase 1 and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. [provided by RefSeq Jul 2008]

Gene Symbol:   Stk10
Gene Name:   Serine/threonine kinase 10
Aliases:   Gek1, Lok, mKIAA4026
RefSeq:   NC_000077.6 NT_039515.7
Ensembl:   ENSMUSG00000020272
Entrez:   20868
UniGene:   Mm.8235
Chromosome Mapping:   11 A4|11 16.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.940000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download