PrimePCR™ Probe Assay: C1qtnf5, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043669
Assay Design:   exonic
Chromosome Location:   9:44107760-44107878question
Amplicon Length:   89
Splice Variants Targeted:   ENSMUST00000113733 ENSMUST00000027358 ENSMUST00000113732 ENSMUST00000022105 ENSMUST00000109680 ENSMUST00000109679 ENSMUST00000087050 ENSMUST00000168787 ENSMUST00000079924 ENSMUST00000149557 ENSMUST00000153710 ENSMUST00000034842 ENSMUST00000034226 ENSMUST00000033737 ENSMUST00000077243 ENSMUST00000161381 ENSMUST00000034654 ENSMUST00000114816 ENSMUST00000114821 ENSMUST00000152956 ENSMUST00000114815 ENSMUST00000114818 ENSMUST00000174525 ENSMUST00000042194 ENSMUST00000023714 ENSMUST00000042615 ENSMUS

Gene Information

The protein encoded by this gene is a member of the C1q/tumor necrosis factor superfamily. This family member is a secretory protein that functions in eye development. Mutations in this gene are thought to underlie the pathophysiology of late-onset retinal degeneration (L-ORD) and early-onset long anterior zonules (LAZ). Bicistronic transcripts composed of the coding sequences for this gene (C1qtnf5) and the membrane-type frizzled-related protein gene (Mfrp) have been identified and the resulting products can interact with each other. Co-transcription of C1qtnf5 and Mfrp has been observed in both human and mouse. Alternative splicing results in multiple transcript variants. [provided by RefSeq Jun 2010]

Gene Symbol:   C1qtnf5
Gene Name:   C1q and tumor necrosis factor related protein 5
Aliases:   Ctrp5
RefSeq:   NC_000075.6 NT_039472.8
Ensembl:   ENSMUSG00000079592
Entrez:   235312
UniGene:   Mm.227110
Chromosome Mapping:   9 A5.2|9 25.5 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 40.980000
Efficiency 83

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download