PrimePCR™ Probe Assay: Vmn1r206, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043507
Assay Design:   exonic
Chromosome Location:   13:22620378-22620506question
Amplicon Length:   99
Splice Variants Targeted:   ENSMUST00000091739 ENSMUST00000075055 ENSMUST00000072369 ENSMUST00000091735 ENSMUST00000091734 ENSMUST00000091732 ENSMUST00000091731 ENSMUST00000091729 ENSMUST00000074324 ENSMUST00000110445 ENSMUST00000124841 ENSMUST00000095961 ENSMUST00000072044 ENSMUST00000073728 ENSMUST00000074252 ENSMUST00000091721 ENSMUST00000076180 ENSMUST00000072385

Gene Information

Description Not Available

Gene Symbol:   Vmn1r206
Gene Name:   Vomeronasal 1 receptor 206
Aliases:   V1rh7
RefSeq:   NC_000079.6 NT_039578.8
Ensembl:   ENSMUSG00000071495
Entrez:   171250
UniGene:   Mm.377205
Chromosome Mapping:   13 A3.1|13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.660000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download