PrimePCR™ Probe Assay: Psmd11, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043019
Assay Design:   exonic
Chromosome Location:   11:80462611-80465043question
Amplicon Length:   60
Splice Variants Targeted:   ENSMUST00000140364 ENSMUST00000174552 ENSMUST00000169960 ENSMUST00000166299 ENSMUST00000034308 ENSMUST00000176860 ENSMUST00000114354 ENSMUST00000114352 ENSMUST00000068286 ENSMUST00000114360 ENSMUST00000033769 ENSMUST00000148317 ENSMUST00000114353 ENSMUST00000101458 ENSMUST00000138397 ENSMUST00000126583 ENSMUST00000112292 ENSMUST00000086599 ENSMUST00000161610 ENSMUST00000108775 ENSMUST00000163193 ENSMUST00000047697 ENSMUST00000020161 ENSMUST00000173938 ENSMUST00000017572 ENSMUST00000148895 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Psmd11
Gene Name:   Proteasome (prosome, macropain) 26S subunit, non-ATPase, 11
Aliases:   1700089D09Rik, 1810019E17Rik, 2610024G20Rik, 2810055C24Rik, C78232, P44.5, S9
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000017428
Entrez:   69077
UniGene:   Mm.260539
Chromosome Mapping:   11 B5|11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 38.460000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download