PrimePCR™ Probe Assay: TXNDC5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TXNDC5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TXNDC5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041146
Assay Design:   Intron-spanning
Chromosome Location:   6:7884679-7889035question
Amplicon Length:   194
Splice Variants Targeted:   ENST00000372050 ENST00000372047 ENST00000324071 ENST00000506607 ENST00000376300 ENST00000319432 ENST00000262301 ENST00000543238 ENST00000399843 ENST00000327979 ENST00000535356 ENST00000045083 ENST00000379757 ENST00000473453 ENST00000539054 ENST00000330503 ENST00000507955 ENST00000502798 ENST00000540638 ENST00000544123 ENST00000400111 ENST00000369547 ENST00000393296 ENST00000399312

Gene Information

This gene encodes a protein-disulfide isomerase. Its expression is induced by hypoxia and its role may be to protect hypoxic cells from apoptosis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring upstream MUTED (muted homolog) gene. [provided by RefSeq Dec 2010]

Gene Symbol:   TXNDC5
Gene Name:   thioredoxin domain containing 5 (endoplasmic reticulum)
Aliases:   ENDOPDI, ERP46, HCC-2, MGC3178, PDIA15, STRF8, UNQ364
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000239264
Entrez:   81567
Chromosome Mapping:   6p24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.970000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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