PrimePCR™ Probe Assay: HAUS7, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041084
Assay Design:   Intron-spanning
Chromosome Location:   X:152713333-152719938question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000330912 ENST00000338525 ENST00000334497 ENST00000370232 ENST00000370211 ENST00000435662 ENST00000370219 ENST00000370212 ENST00000421080 ENST00000303015 ENST00000520439 ENST00000597970 ENST00000596566 ENST00000595172 ENST00000595261 ENST00000597897 ENST00000598817 ENST00000599647 ENST00000595628 ENST00000600844

Gene Information

This gene encodes a protein identified by interaction with ubiquitin C-terminal hydrolase 37 which functions to edit polyubiquitin chains on ubiquitinated substrates. This protein is a subunit of the multisubunit augmin complex which regulates centrosome and mitotic spindle integrity and is necessary for the completion of cytokinesis. [provided by RefSeq Jul 2009]

Gene Symbol:   HAUS7
Gene Name:   HAUS augmin-like complex, subunit 7
Aliases:   HSXQ28ORF, UCHL5IP, UIP1
RefSeq:   NC_000023.10 NG_012688.1 NT_167198.1
Ensembl:   ENSG00000213397
Entrez:   55559
UniGene:   Hs.170835
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.940000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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