PrimePCR™ Probe Assay: TRIM15, Human


Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041060
Assay Design:   Intron-spanning
Chromosome Location:   6:30136266-30139619question
Amplicon Length:   194
Splice Variants Targeted:   ENST00000546888 ENST00000440260 ENST00000430090 ENST00000355703 ENST00000414873 ENST00000425255 ENST00000552062 ENST00000427091 ENST00000444157 ENST00000550694 ENST00000429524 ENST00000424597 ENST00000550702 ENST00000376694 ENST00000433744 ENST00000421984 ENST00000423220 ENST00000548732 ENST00000431059 ENST00000426765 ENST00000548653 ENST00000259930 ENST00000427952 ENST00000550969 ENST00000296137 ENST00000535325 ENST00000449058 ENST00000411850 ENST00000425375 ENST00000436462 ENST00000447733 ENST

Gene Information

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains a RING a B-box type 1 and a B-box type 2 and a coiled-coil region. The protein localizes to the cytoplasm. Alternatively spliced transcript variants have been described but their biological validity has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   TRIM15
Gene Name:   tripartite motif containing 15
Aliases:   RNF93, ZNF178, ZNFB7
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204610
Entrez:   89870
UniGene:   Hs.591789
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.090000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download