PrimePCR™ Probe Assay: CXorf40B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CXorf40B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CXorf40B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041005
Assay Design:   Intron-spanning
Chromosome Location:   X:149100877-149101920question
Amplicon Length:   160
Splice Variants Targeted:   ENST00000395564 ENST00000341253 ENST00000286744 ENST00000567476 ENST00000450602 ENST00000441248 ENST00000393985 ENST00000423421 ENST00000423540 ENST00000434353 ENST00000514208 ENST00000428236 ENST00000422892 ENST00000359293 ENST00000462691 ENST00000370406 ENST00000355203 ENST00000370404 ENST00000595814 ENST00000601066 ENST00000597294 ENST00000598640 ENST00000596374 ENST00000597532 ENST00000594483 ENST00000601621 ENST00000598998 ENST00000593915 ENST00000595555 ENST00000601122 ENST00000596222 ENST

Gene Information

Description Not Available

Gene Symbol:   CXorf40B
Gene Name:   chromosome X open reading frame 40B
Aliases:   Not Available
RefSeq:   NC_000023.10 NT_167198.1
Ensembl:   ENSG00000197021
Entrez:   541578
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.700000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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