PrimePCR™ Probe Assay: FDPS, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: FDPS, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FDPS, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040828
Assay Design:   Intron-spanning
Chromosome Location:   1:155282150-155288013question
Amplicon Length:   142
Splice Variants Targeted:   ENST00000595962 ENST00000356458 ENST00000446533 ENST00000272203 ENST00000414478 ENST00000368356 ENST00000356657 ENST00000447866 ENST00000361684 ENST00000361906

Gene Information

This gene encodes an enzyme that catalyzes the production of geranyl pyrophosphate and farnesyl pyrophosphate from isopentenyl pyrophosphate and dimethylallyl pyrophosphate. The resulting product farnesyl pyrophosphate is a key intermediate in cholesterol and sterol biosynthesis a substrate for protein farnesylation and geranylgeranylation and a ligand or agonist for certain hormone receptors and growth receptors. Drugs that inhibit this enzyme prevent the post-translational modifications of small GTPases and have been used to treat diseases related to bone resorption. Multiple pseudogenes have been found on chromosomes 1 7 14 15 21 and X. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Oct 2008]

Gene Symbol:   FDPS
Gene Name:   farnesyl diphosphate synthase
Aliases:   FPPS, FPS
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000160752
Entrez:   2224
Chromosome Mapping:   1q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.790000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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