PrimePCR™ Probe Assay: GPR89A, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040715
Assay Design:   Intron-spanning
Chromosome Location:   1:145804300-145811425question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000313835 ENST00000534502 ENST00000462900 ENST00000454423 ENST00000314163 ENST00000582624 ENST00000582339 ENST00000583232 ENST00000577475 ENST00000580001

Gene Information

GPR89A is a nearly identical copy of the GPR89B gene (MIM 612806).[supplied by OMIM Jun 2009]

Gene Symbol:   GPR89A
Gene Name:   G protein-coupled receptor 89A
Aliases:   FLJ10492, FLJ11696, FLJ36909, FLJ79270, GPR89B, MGC688, MGC87324, UNQ192
RefSeq:   NC_000001.10 NT_167185.1
Ensembl:   ENSG00000117262
Entrez:   653519
UniGene:   Hs.504320
Chromosome Mapping:   1q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.550000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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