PrimePCR™ Probe Assay: TBC1D3H, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TBC1D3H, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TBC1D3H, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040224
Assay Design:   Intron-spanning
Chromosome Location:   17:34750396-34752353question
Amplicon Length:   137
Splice Variants Targeted:   ENST00000453547 ENST00000266058 ENST00000371070 ENST00000290573 ENST00000409174 ENST00000429084 ENST00000359593 ENST00000421755 ENST00000422582 ENST00000329517 ENST00000300113 ENST00000454519 ENST00000398801 ENST00000487903 ENST00000418678 ENST00000375630 ENST00000375645 ENST00000283558 ENST00000336331 ENST00000457979 ENST00000308078 ENST00000451448 ENST00000394359 ENST00000455054 ENST00000535446 ENST00000400684 ENST00000394453 ENST00000592614 ENST00000341264 ENST00000330458 ENST00000535592 ENST

Gene Information

Description Not Available

Gene Symbol:   TBC1D3H
Gene Name:   TBC1 domain family, member 3H
Aliases:   Not Available
RefSeq:   NC_000017.10 NT_010783.15 NW_003315949.1
Ensembl:   ENSG00000242384
Entrez:   729877
Chromosome Mapping:   17q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.000000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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