PrimePCR™ Probe Assay: LRRC37A2, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040210
Assay Design:   Intron-spanning
Chromosome Location:   17:44592625-44597622question
Amplicon Length:   150
Splice Variants Targeted:   ENST00000547334 ENST00000547001 ENST00000549105 ENST00000597888 ENST00000600392 ENST00000552021 ENST00000543378 ENST00000327564 ENST00000584368 ENST00000341671 ENST00000394713 ENST00000495880 ENST00000296483 ENST00000584306 ENST00000584788 ENST00000319651 ENST00000393465 ENST00000320254 ENST00000576629 ENST00000333412 ENST00000591674 ENST00000341156 ENST00000262809 ENST00000340648 ENST00000341552 ENST00000441968 ENST00000453220 ENST00000433935 ENST00000592813 ENST00000573751 ENST00000269289 ENST

Gene Information

Description Not Available

Gene Symbol:   LRRC37A2
Gene Name:   leucine rich repeat containing 37, member A2
Aliases:   FLJ45049
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000238083
Entrez:   474170
UniGene:   Hs.646568
Chromosome Mapping:   17q21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996000
y-intercept 35.920000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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