PrimePCR™ Probe Assay: ZFP57, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ZFP57, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZFP57, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040080
Assay Design:   Intron-spanning
Chromosome Location:   6:29641461-29643220question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000488757 ENST00000376883 ENST00000376881 ENST00000437216 ENST00000548574 ENST00000547542 ENST00000416974 ENST00000552809 ENST00000552898 ENST00000435906 ENST00000549167 ENST00000549501 ENST00000383628 ENST00000548769 ENST00000552987 ENST00000448114 ENST00000548337 ENST00000548001 ENST00000446005 ENST00000553137 ENST00000549312

Gene Information

The protein encoded by this gene is a zinc finger protein containing a KRAB domain. Studies in mouse suggest that this protein may function as a transcriptional repressor. Mutations in this gene have been associated with transient neonatal diabetes mellitus type 1 (TNDM1).[provided by RefSeq Sep 2009]

Gene Symbol:   ZFP57
Gene Name:   zinc finger protein 57 homolog (mouse)
Aliases:   C6orf40, TNDM1, ZNF698, bA145L22, bA145L22.2
RefSeq:   NC_000006.11 NG_013045.1 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204644
Entrez:   346171
Chromosome Mapping:   6p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.180000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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