PrimePCR™ Probe Assay: MRC1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MRC1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MRC1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039797
Assay Design:   Intron-spanning
Chromosome Location:   10:18122738-18129673question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000569591 ENST00000331429 ENST00000457317 ENST00000239761

Gene Information

The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes including cell-cell recognition serum glycoprotein turnover and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses bacteria and fungi so that they can be neutralized by phagocytic engulfment.[provided by RefSeq Apr 2011]

Gene Symbol:   MRC1
Gene Name:   mannose receptor, C type 1
Aliases:   CD206, CLEC13D, CLEC13DL, MGC181940, MMR, MRC1L1, bA541I19.1
RefSeq:   NC_000010.10 NT_008705.16 NW_003315932.1
Ensembl:   ENSG00000120586
Entrez:   4360
Chromosome Mapping:   10p12.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.780000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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