PrimePCR™ Probe Assay: PGBD1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PGBD1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PGBD1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029847
Assay Design:   Intron-spanning
Chromosome Location:   6:28253408-28264605question
Amplicon Length:   148
Splice Variants Targeted:   ENST00000259883 ENST00000405948

Gene Information

The piggyBac family of proteins found in diverse animals are transposases related to the transposase of the canonical piggyBac transposon from the moth Trichoplusia ni. This family also includes genes in several genomes including human that appear to have been derived from the piggyBac transposons. This gene belongs to the subfamily of piggyBac transposable element derived (PGBD) genes. The PGBD proteins appear to be novel with no obvious relationship to other transposases or other known protein families. This gene product is specifically expressed in the brain however its exact function is not known. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq May 2010]

Gene Symbol:   PGBD1
Gene Name:   piggyBac transposable element derived 1
Aliases:   HUCEP-4, SCAND4, dJ874C20.4
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000137338
Entrez:   84547
Chromosome Mapping:   6p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 35.180000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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