PrimePCR™ Probe Assay: CD79A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029327
Assay Design:   Intron-spanning
Chromosome Location:   19:42383685-42385045question
Amplicon Length:   190
Splice Variants Targeted:   ENST00000221972 ENST00000444740

Gene Information

The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins Ig-alpha and Ig-beta which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-alpha protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   CD79A
Gene Name:   CD79a molecule, immunoglobulin-associated alpha
Aliases:   IGA, MB-1
RefSeq:   NC_000019.9 NG_009619.1 NT_011109.16
Ensembl:   ENSG00000105369
Entrez:   973
Chromosome Mapping:   19q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996600
y-intercept 36.890000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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