PrimePCR™ Probe Assay: GUSB, Human

print
RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:   
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028142
Assay Design:   Intron-spanning
Chromosome Location:   7:65432849-65435331question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000304895 ENST00000421103 ENST00000345660

Gene Information

This gene encodes a hydrolase that degrades glycosaminoglycans including heparan sulfate dermatan sulfate and chondroitin-46-sulfate. The enzyme forms a homotetramer that is localized to the lysosome. Mutations in this gene result in mucopolysaccharidosis type VII. There are many pseudogenes of this locus in the human genome.[provided by RefSeq Nov 2009]

Gene Symbol:   GUSB
Gene Name:   glucuronidase, beta
Aliases:   BG, FLJ39445, MPS7
RefSeq:   NC_000007.13 NG_016197.1 NT_007933.15
Ensembl:   ENSG00000169919
Entrez:   2990
UniGene:   Hs.255230
Chromosome Mapping   7q21.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.230000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.