This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: MUC5B, Human
PrimePCR™ Template for Probe Assay: MUC5B, Human
This gene encodes a member of the mucin family of proteins which are highly glycosylated macromolecular components of mucus secretions. This family member is the major gel-forming mucin in mucus. It is a major contributor to the lubricating and viscoelastic properties of whole saliva normal lung mucus and cervical mucus. This gene has been found to be up-regulated in some human diseases including sinus mucosa of chronic rhinosinusitis (CRS) CRS with nasal polyposis chronic obstructive pulmonary disease (COPD) and H. pylori-associated gastric disease and it may be involved in the pathogenesis of these diseases. [provided by RefSeq Jul 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.