PrimePCR™ Probe Assay: AHSG, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: AHSG, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AHSG, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027935
Assay Design:   Intron-spanning
Chromosome Location:   3:186337646-186338422question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000411641 ENST00000273784 ENST00000541510

Gene Information

Alpha2-HS glycoprotein (AHSG) a glycoprotein present in the serum is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions such as endocytosis brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix and it has therefore been postulated that it participates in the development of the tissues. However its exact significance is still obscure. [provided by RefSeq Jul 2008]

Gene Symbol:   AHSG
Gene Name:   alpha-2-HS-glycoprotein
Aliases:   A2HS, AHS, FETUA, HSGA
RefSeq:   NC_000003.11 NG_011436.1 NT_005612.16
Ensembl:   ENSG00000145192
Entrez:   197
Chromosome Mapping:   3q27

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 34.420000
Efficiency 108

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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