This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029001
PrimePCR™ PreAmp for SYBR® Green Assay: SEMA3F, Human
PrimePCR™ Template for SYBR® Green Assay: SEMA3F, Human
The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal a 500-amino acid sema domain and 16 conserved cysteine residues (Kolodkin et al. 1993 [PubMed 8269517]). Sequence comparisons have grouped the secreted semaphorins into 3 general classes all of which also have an immunoglobulin domain. The semaphorin III family consisting of human semaphorin III (SEMA3A; MIM 603961) chicken collapsin and mouse semaphorins A D and E all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al. 1993 [PubMed 8402908]) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al. 1995 [PubMed 7637782]) a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168). SEMA3F is a secreted member of the semaphorin III family.[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.