Gene:  SEMA3F, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal a 500-amino acid sema domain and 16 conserved cysteine residues (Kolodkin et al. 1993 [PubMed 8269517]). Sequence comparisons have grouped the secreted semaphorins into 3 general classes all of which also have an immunoglobulin domain. The semaphorin III family consisting of human semaphorin III (SEMA3A; MIM 603961) chicken collapsin and mouse semaphorins A D and E all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al. 1993 [PubMed 8402908]) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al. 1995 [PubMed 7637782]) a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168). SEMA3F is a secreted member of the semaphorin III family.[supplied by OMIM Mar 2008]

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PrimePCR™ SYBR® Green Assay: SEMA3F, Human
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0013705
Info:   Same primer pair as used in probe assay qHsaCIP0029001
List Price:    $130.00
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PrimePCR™ Probe Assay: SEMA3F, Human
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0029001
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: SEMA3F, Human
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5019526
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0013705; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: SEMA3F, Human
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5051628
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0029001; Intron-spanning
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: SEMA3F, Human
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5051629
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0029001; Intron-spanning
 
Fluorophore:   HEX
List Price:    $195.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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