This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027765
PrimePCR™ PreAmp for SYBR® Green Assay: TRAF2, Human
PrimePCR™ Template for SYBR® Green Assay: TRAF2, Human
The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs) and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD a TNF receptor associated apoptotic signal transducer ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1 an apoptosis inhibitor possessing ubiquitin ligase activity can unbiquitinate and induce the degradation of this protein and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene but the biological validity of only one transcript has been determined. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.