PrimePCR™ SYBR® Green Assay: LDB2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027720

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LDB2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LDB2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010761
Assay Design:   Intron-spanning
Chromosome Location:   4:16590409-16597427question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000515064 ENST00000441778 ENST00000304523 ENST00000502640 ENST00000507464 ENST00000506732 ENST00000503178

Gene Information

Genes encoding LIM domain-binding factors were initially isolated in a screen for proteins that physically interact with the LIM domains of nuclear proteins (summarized by Semina et al. 1998 [PubMed 9799849]). These proteins such as the one encoded by the LDB2 gene are capable of binding to a variety of transcription factors and are likely to function at enhancers to bring together diverse transcription factors and form higher order activation complexes or to block formation of such complexes (Jurata and Gill 1997 [PubMed 9315627]). The family of genes encoding LIM domain-binding factors includes 2 members isolated from the mouse Clim1 (Bach et al. 1997 [PubMed 9192866]) and Clim2/Lbd1/Nli (Agulnick et al. 1996 [PubMed 8918878]; Jurata et al. 1996 [PubMed 8876198]; Bach et al. 1997 [PubMed 9192866]) and their homologs cloned from the frog chicken and fly. The fact that LIM domain-binding factors are likely to be involved in the coordination of the transcriptional activity of many diverse factors might implicate them in human phenotypes characterized by multiple affected sites.[supplied by OMIM Jul 2010]

Gene Symbol:   LDB2
Gene Name:   LIM domain binding 2
Aliases:   CLIM1, LDB1
RefSeq:   NC_000004.11 NT_006316.16
Ensembl:   ENSG00000169744
Entrez:   9079
Chromosome Mapping:   4p16

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.600000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download