This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027720
PrimePCR™ PreAmp for SYBR® Green Assay: LDB2, Human
PrimePCR™ Template for SYBR® Green Assay: LDB2, Human
Genes encoding LIM domain-binding factors were initially isolated in a screen for proteins that physically interact with the LIM domains of nuclear proteins (summarized by Semina et al. 1998 [PubMed 9799849]). These proteins such as the one encoded by the LDB2 gene are capable of binding to a variety of transcription factors and are likely to function at enhancers to bring together diverse transcription factors and form higher order activation complexes or to block formation of such complexes (Jurata and Gill 1997 [PubMed 9315627]). The family of genes encoding LIM domain-binding factors includes 2 members isolated from the mouse Clim1 (Bach et al. 1997 [PubMed 9192866]) and Clim2/Lbd1/Nli (Agulnick et al. 1996 [PubMed 8918878]; Jurata et al. 1996 [PubMed 8876198]; Bach et al. 1997 [PubMed 9192866]) and their homologs cloned from the frog chicken and fly. The fact that LIM domain-binding factors are likely to be involved in the coordination of the transcriptional activity of many diverse factors might implicate them in human phenotypes characterized by multiple affected sites.[supplied by OMIM Jul 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.